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Adapter Template Oligo-Mediated Sequencing

ATOM-Seq: a unique alternative to ligation-based DNA capture

A reimagining of how to process patient samples.

 

Designed to address the challenges of processing limited or poor-quality clinical samples, by combining the advantages and overcoming limitations of other common library preparation approaches.

ATOM-Seq Technological Overview

The patented ATOM-Seq capture chemistry is simple and elegant. Treating the sample nucleic acids as a primer, their 3' ends are first annealed to a synthetic template known as an Adaptor Template Oligo, before being captured by the extension of these 3' ends by a polymerase.

 

This ATOM-Seq capture method of nucleic acids incorporates both true Unique Molecular Identifiers (UMIs) and a universal primer site directly onto available 3' ends of every original sample molecule.

Ligation free capture chemistry combines advantages of common approaches while avoiding their disadvantages. 

Capture more of your patient sample with ATOM-Seq which captures all single stranded, double-stranded or nicked DNA of any length. 

See comparison data here

Better performance with limited samples 

with optimisation to maximise the amount of information which can be obtained from precious patient samples

Process even the most degraded and damaged samples, with resilient workflows able to process even the lowest quality sample.

Detect even the rarest clinical signatures by using both UMIs and unique, error-reducing workflow optimisations

Simple, single-day protocols leveraging the inherent simplicity and efficiency of DNA polymerases

Minimal hands-on time, due to few bead purifications and effortless reaction set up

No specialist equipment required, allowing for rapid implementation in all laboratory settings

Dunwell, T.L., et al. Adaptor Template Oligo-Mediated Sequencing (ATOM-Seq) is a new ultra-sensitive UMI-based NGS library preparation technology for use with cfDNA and cfRNA. Sci Rep 11, 3138 (2021).

ATOM-Seq Workflows

Targeted cfDNA

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For identification of single nucleotide variants, insertions, and deletions from limited cell-free DNA

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Workflows are compatible with plasma and urine samples​

Targeted FFPE DNA

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For identification of single nucleotide variants,

insertions, deletions, copy number variation and MSI

from even poor quality FFPE-preserved DNA

Targeted FFPE RNA

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For the identification of known and novel gene fusions from poor quality RNA samples

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Workflows are also compatible with detection of single nucleotide variants, insertions, deletions, and relative RNA expression

Whole-sample​

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For capturing all DNA from a sample to identify all genetic and/or epi-genetic signatures from limited cell-free or FFPE-preserved DNA

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Customised solutions​

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The ATOM-Seq chemistry has solutions to answer almost any clinical or research questions, even beyond those listed above

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If our off-the-shelf products don't match your needs or you are looking for something more unique, please contact us to discuss your project and how we can support you

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