XCeloSeq
Sarcoma Fusion Kit
®
NGS Library Preparation kit for use with FFPE RNA
Use ATOM-Seq to capture and enrich for gene fusions from 26 genes which are frequently involved in chromosomal rearrangements in sarcomas. Amplification targeted by only single primers, each amplifying a conserved fusion partner, ensures both known and unknown fusion events are detected. Suitable for even highly damaged FFPE RNA samples.
Product Details
Specifications
Home > ATOM-Seq Capture Chemistry > RNA Enrichment Workflow > XCeloSeq Sarcoma Fusion Kit
* Higher input masses will provide better sensitivities. Very poor quality material, RIN < 3 and DV200 < 20% can increase input mass to 300 ng
# 8 Additional primers targeting controls are also included.
Target Genes
Targets fusions across 54 genes most frequently involved in generating fusions across sarcomas
Benefits
Simple, single-day protocols leveraging the inherent simplicity and efficiency of DNA polymerases
Process even the lowest quality RNA by capturing both single- and double-stranded cDNA from highly degraded FFPE RNA samples
Identify all fusions (known and unknown) and exon skipping by using a single targeting primer for each conserved exon
Unique Dual Indexes
Unique Dual Indexes (UDIs) are required to be purchased and are sold separately
Please contact us to discuss additional index options.
Resources
Product guide and protocol
For Research Use Only (RUO).
Not for use in diagnostic procedures. Not intended to be used for treatment of human or animal diseases.